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Remove as much culture media as possible, to reduce dilution of cryostor® solution Remove as much culture media as possible, to reduce dilution of cryostor solution Calculate volume of cs5 resuspension (recommended cryopreservation concentration range

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Remove conical from centrifuge and aspirate the supernatant without disturbing the pellet Centrifuge cells to obtain cell pellet Gently resuspend the cell pellet in cryostor5 and mix until homogenous cell solution is obtained

The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis

Cryostor® cs2,cs5 and cs10 freeze media usage and cryopreservation protocol place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)