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Eps are the construction material of bacterial settlements and either remain attached to the cell's outer surface, or are secreted into its growth medium Bacterial biofilms are highly structured, functional, specific and coordinated. These compounds are important in biofilm formation and cells' attachment to surfaces

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Eps constitute 50% to 90% of a biofilm's total organic matter Eps facilitates intercellular communication, protects cells from chemical damage, provides oxygen diffusion, releases extracellular enzymes for nutrition and, in turn, stimulates the spread of bacteria within the biofilm (costa et al., 2018 One of the key elements in the establishment and maintenance of the biofilm structure and properties is the extracellular matrix

The extracellular matrix is composed of water and extracellular polymeric substances (eps)

Primarily polysaccharides, proteins and dna. Extracellular polymeric substances (eps) are responsible for establishing the structural and functional integrity of microbial biofilms The primary constituents of eps discharged into the environment by microorganisms consist of polysaccharides, proteins, lipids, and extracellular dna (edna). Eps are biopolymers of microbial origin in which biofilm microorganisms are embedded

In fact, archaeal, bacterial, and eukaryotic microbes produce the biopolymers Contrary to common belief, eps are certainly more than only polysaccharides. The 1 h nmr analysis of eps from p Coli biofilms over 24 and 72 h provided valuable insights into the chemical changes associated with biofilm maturation.